Protein Phosphorylation

Reversible phosphorylation is one of the major mechanisms of controlling protein activity in all eukaryotic cells. This new edition of Protein Phosphorylation: A Practical Approach provides a comprehensive description of current methods used to study protein phosphorylation and the kinases andphosphatases which catalyse it. It includes protocols for studying phosphorylation in intact cells; analysis of signal transduction pathways, kinase specificity, and kinase interactions; assay and purification of kinases and phosphatases; and identification of substrates. Also covered are cloningand expression protocols and advice on the crystallization of kinases and phosphatases.Hardie, D. Grahame is the author of 'Protein Phosphorylation' with ISBN 9780199633067 and ISBN 0199633061.